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Karyological Study of Siberian Larch Species Larix sibirica and Larix gmelinii in Taimyr

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Abstract

A comparative study of karyotypes of two Siberian species of larch—L. sibirica and L. gmelinii—growing in the zone of forest-tundra on the Taimyr Peninsula was carried out. The number of chromosomes has been analyzed and the increase in chromosomal instability in larches in forest-tundra conditions has been shown. The size and morphology of chromosomes and the localization and frequency of secondary constrictions in chromosomes of L. sibirica and L. gmelinii were estimated. The parameters of chromosomes in polyploid cells of L. gmelinii were studied. The asymmetry of karyotypes of L. sibirica and L. gmelinii was analyzed. The spectrum and frequency of chromosome rearrangements in L. sibirica and L. gmelinii were studied. Using the data of the karyological analysis, the issues of differentiation and adaptation of the investigated larch species in Taimyr are discussed.

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REFERENCES

  1. Bobrov, E.G., Forest-Forming Coniferous USSR, Leningrad: Nauka, 1978.

    Google Scholar 

  2. Koropachinskiy, I.Yu. and Milyutin, L.I., Natural hybridization of woody plants, Novosibirsk: Academic Publishing House Geo, 2006.

    Google Scholar 

  3. Dylis, N.V., Siberian Larch, Sukachev, V.N., Ed., Moscow: Publ. House of the Moscow Society of Naturalists, 1947.

    Google Scholar 

  4. Putenikhin, V.P., Farukshina, G.G., and Shigapov, Z.Kh., Sukachev’s Larch in the Urals: Variability and Population-Genetic Structure, Moscow: Nauka, 2004.

    Google Scholar 

  5. Milyutin, L.I., Biodiversity of larch of Russia, Khvoin. Boreal. Zony. Larch, 2003, no. 1, pp. 6–9.

  6. Semerikov, V.L. and Lascoux, M., Nuclear and cytoplasmic variation within and between Eurasian Larix (Pinaceae) species, Am. J. Bot., 2003, vol. 90, pp. 1113–1123. https://doi.org/10.3732/ajb.90.8.1113

    Article  CAS  PubMed  Google Scholar 

  7. Farjon, A., World Checklist and Bibliography of Conifers, Kew: The Royal Botanic Garden, 2001.

    Google Scholar 

  8. Dylis, N.V., Larch of Siberia and the Far East, Moscow, 1961.

    Google Scholar 

  9. Abaimov, A.P., Karpel, B.A., and Koropachinsky, I.Yu., On the limits of areas of Siberian larch species, Bot. Zh., 1980, vol. 65, no. 1, pp. 118–120.

    Google Scholar 

  10. Polezhaeva, M.A., Lascoux, M., and Semerikov, V.L., Cytoplasmic DNA variation and biogeography of Larix Mill. in Northern Asia, Mol. Ecol., 2010, vol. 19, pp. 1239–1252. https://doi.org/10.1111/j.1365-294X.2010.04552.x

    Article  Google Scholar 

  11. Oreshkova, N.V., Belokon, M.M., and Jamiyansuren, S., Genetic diversity, population structure, and differentiation of Siberian larch, Gmelin larch, and Cajander larch on SSR-marker data, Russ. J. Gen. Genet., 2013, vol. 49, no. 2, pp. 178–186. https://doi.org/10.1134/S1022795412120095

    Article  CAS  Google Scholar 

  12. Kruklis, M.V. and Milyutin, L.I., Larch of Chekanovsky, Moscow: Nauka, 1977.

    Google Scholar 

  13. Efremov, S.P., Sedelnikova, T.S., and Pimenov, A.V., Morphological features of cones of Siberian larch in wetland and dry land conditions, Khvoin. Boreal. Zony, 2006, vol. 23, no. 2, pp. 223–227.

    Google Scholar 

  14. Barchenkov, A.P., Intraspecific variability of the Siberian Larch Larix sibirica Ledeb. seed scales, Sib. Lesn. Zh., 2016, no. 6, pp. 126–132. https://doi.org/10.15372/SJFS20160612

  15. Oreshkova, N.V., Larionova, A.Ya., Milyutin, L.I., and Abaimov, A.P., Genetic diversity, structure and differentiation of Gmelin larch (Larix gmelinii (Rupr.) Rupr.) populations from central Evenkia and Eastern Zabaikalje, Eurasian J. Forest Res., 2006, vol. 9, no. 1, pp. 1–8.

    Google Scholar 

  16. Semerikov, V.L., Semerikova, S.A., Polezhaeva, M.A., Kosintsev, P.A., and Lascoux, M., Southern montane populations did not contribute to the recolonization of West Siberian plain by Siberian larch (Larix sibirica): a range-wide analysis of cytoplasmic markers, Mol. Ecol., 2013, vol. 22, no. 19, pp. 4958–71. https://doi.org/10.1111/mec.12433

    Article  CAS  PubMed  Google Scholar 

  17. Kruse, S., Epp, L.S., Wieczorec, M., Pestryakova, L.A., Stoof-Leichsenring, K.R., and Herzscuh, U., High gene flow and complex treeline dynamics of Larix Mill. stands on the Taymyr Peninsula (north-central Siberia) revealed by nuclear microsatellites, Tree Genet. Genom., 2018, vol. 14, no. 19. https://doi.org/10.1007/s11295-018-1235-3

  18. Larch Biodiversity of Asian Russia, Yefremov, S.P. and Milyutin, L.I., Eds., Novosibirsk: Academic Publishing House Geo, 2010.

    Google Scholar 

  19. Vetrova, V.P., Oreshkova, N.V., and Sinelnikova, N.V., Differentiation of Larix cajanderi (Pinaceae) populations on cone scales morphology and DNAmarkers in the east of the range, Bot. Zh., 2016, vol. 101, no. 9, pp. 993–1007.

    Article  Google Scholar 

  20. Muratova, E.N. and Chubukina, N.E., Karyological study of Sukachev’s larch (Larix sukaczewii N. Dyl.): nucleolar regions and structural rearrangements, Tsitol. Genet., 1985, vol. 19, no. 6, pp. 419–425.

    Google Scholar 

  21. Muratova, E.N., Karyological study of Larix sibirica (Pinaceae) in various parts of the range, Bot. Zh., 1991, vol. 76, no. 11, pp. 1586–1595.

    Google Scholar 

  22. Muratova, E.N., Chromosomal polymorphism in natural populations of Gmelin larch Larix gmelinii (Rupr.) Rupr., Tsitol. Genet., 1994, vol. 28, no. 4, pp. 14–22.

    Google Scholar 

  23. Sedelnikova, T.S. and Pimenov, A.V., Karyological study of bog and dry-valley populations of Larix sibirica (Pinaceae) from Western Siberia, Bot. Zh., 2005, vol. 90, no. 4, pp. 582–593.

    Google Scholar 

  24. Sedelnikova, T.S. and Pimenov, A.V., Chromosomal mutations in Siberian larch (Larix sibirica Ledeb.) on Taimyr Peninsula, Biol. Bull., 2007, vol. 34, no. 2, pp. 198–201. https://doi.org/10.1134/s1062359007020136

    Article  Google Scholar 

  25. Muratova, E.N., Karpyuk, T.V., Vladimirova, O.S., Sizykh, O.A., and Kvitko, O.V., A cytological study of Siberian larch in anthropogenically disturbed areas of the city of Krasnoyarsk and its vicinity, Vestn. Ekol. Lesoved. Landshaftoved., 2008, no. 9, pp. 99–108.

  26. Kvitko, O.V., Muratova, E.N., Syzikh, O.A., and Vladimirova, O.S., Chromosome numbers of some conifer species, Bot. Zh., 2009, vol. 94, no. 2, pp. 305–307.

    Google Scholar 

  27. Reference Book on the Climate of the USSR, vol. 21: Krasnoyarsk Territory and Tuva ASSR, Leningrad: Gidrometeoizdat, 1973.

  28. State Report “On the State and Protection of the Environment in the Krasnoyarsk Territory in 2016,” Krasnoyarsk, 2017, pp. 157–164.

  29. Kirdyanov, A.V., Myglan, V.S., Pimenov, A.V., Knorre, A.A., Ekart, A.K., and Vaganov, E.A., Die-off dynamics of Siberian larch under the impact of pollutants emitted by Norilsk enterprises, Contemp. Probl. Ecol., 2014, vol. 7, no. 6, pp. 679–684. https://doi.org/10.1134/s1995425514060055

    Article  Google Scholar 

  30. Lima-de-Faria, A., Classification of genes, rearrangements and chromosomes according to the chromosome field, Hereditas, 1980, vol. 93, pp. 1–46.

    Article  CAS  PubMed  Google Scholar 

  31. Levan, A., Fredga, K., and Sandberg, A.A., Nomenclature for centromeric position on chromosomes, Hereditas, 1964, vol. 52, pp. 201–20.

    Article  Google Scholar 

  32. Stebbins, G.L., Chromosomal Evolution in Higher Plants, London: Edward Arnold Publ. Ltd., 1971.

    Google Scholar 

  33. Peruzzi, L. and Eroglu, H.E., Karyotype asymmetry: again, how to measure and what to measure, Comp. Cytogenet., 2013, vol. 7, no. 1, pp. 1–9. https://doi.org/10.3897/CompCytogen.v7i1.4431

    Article  PubMed  PubMed Central  Google Scholar 

  34. Huziwara, Y., Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster, Am. J. Bot., 1962, vol. 49, pp. 116–119.

    Article  Google Scholar 

  35. Arano, H. and Saito, H., Cytological studies in family Umbelliferae. 5. Karyotypes of seven species in subtribe Seselinae, La Kromosomo, 1980, vol. 2, pp. 471–480.

    Google Scholar 

  36. Baeza, C., Schrader, E., Ruiz, E., and Negritto, M., Analisis comparativo del cariotipo en poblaciones de Alstroemeria aurea R. Graham. (Alstroemeriaceae) de Chile, Gayana Bot., 2007, vol. 64, pp. 33–39. https://doi.org/10.1590/s1415-47572010005000012

    Article  Google Scholar 

  37. Romero Zarco, C., A new method for estimating karyotype asymmetry, Taxon, 1986, vol. 35, pp. 526–530.

    Article  Google Scholar 

  38. Sedelnikova, T.S. and Pimenov, A.V., Chromosome numbers of Larix sibirica (Pinaceae) forms in the Shira steppe of the Republic of Khakassia, Bot. Zh., 2017, vol. 102, no. 5, pp. 693–697.

    Article  Google Scholar 

  39. Sedelnikova, T.S. and Pimenov, A.V., Chromosome numbers of Larix (Pinaceae) species in forest–steppe and forest–tundra of Middle Siberia, Bot. Zh., 2017, vol. 102, no. 12, pp. 1694–7.

    Article  Google Scholar 

  40. Zhang, S.-G., Yang, W.H., Han, S.Y., Han, B.T., Li, M.X., and Qi, L.W., Cytogenetic analysis of reciprocal hybrids and their parents between Larix leptolepis and Larix gmelinii: implications for identifying hybrids, Tree Genet. Genom., 2010, no. 6, pp. 405–412. https://doi.org/10.1007/s11295-009-0258-1

  41. Goryachkina, O.V., Badaeva, E.D., Muratova, E.N., and Zelenin, A.V., Molecular cytogenetic analysis of Siberian Larix species by fluorescence in situ hybridization, Plant Syst. Evol., 2013, vol. 299, pp. 471–479. https://doi.org/10.1007/s00606-012-0737-y

    Article  Google Scholar 

  42. Larsen, S. and Westergaard, M., Contribution to the cytogenetics of forest trees. I. A triploid hybrid between Larix decidua Miller and Larix occidentalis Nutt, J. Genet., 1938, vol. 3, pp. 523–530.

    Article  Google Scholar 

  43. Illies, Z.M., Two aneuploid generations of larch hybrids deriving from colchicine induced Larix sp., in Second World Consult. on Forest Tree Breeding, Washington, August 7–16, 1969, pp. 6–10.

  44. Butorina, A.K., Deryuzhkin, R.I., Muraia, L.S., Sivo-lapov, A.I., and Idjomah, J., Cytological features of heterotic larch, Lesovedenie, 1987, no. 4, pp. 82–86.

  45. Kunakh, V.A., Ontogenetic plasticity of the genome as the basis of plant adaptability, in Zhebrakovsky Readings. III. “Transformation of Genomes,” Minsk: Inst. Genet. Cytol., Natl. Acad. Sci. Belarus, 2011.

    Google Scholar 

  46. Brochmann, C., Brysting, A.K., Alsos, I.G., Borgen, L., Grundt, H.H., Scheen, A.-C., and Elven, R., Polyploidy in arctic plants, Biol. J. Linn. Soc., 2004, vol. 82, pp. 521–536. https://doi.org/10.1111/j.1095-8312.2004.00337.x

    Article  Google Scholar 

  47. Parnikoza, I., Kozeretska, I., and Kunakh, V., Vascular plants of the Maritime Antarctic: origin and adaptation, Am. J. Pl. Sci., 2011, vol. 2, no. 3, pp. 381–395. https://doi.org/10.4236/ajps.2011.23044

    Article  Google Scholar 

  48. Navrotska, D.O., Twardovska, M.O., Andreev, I.O., Parnikoza, I.Yu., Betekhtin, A.A., Zahrychuk, O.M., Drobyk, N.M., Hasterok, R., and Kunakh, V.A., New forms of chromosome polymorphism in Deschampsia antarctica Desv. from the Argentine Islands of the Maritime Antarctic region, Ukr. Antarkt. Zh., 2014, no. 13, pp. 185–191. http://nbuv.gov.ua/UJRN/uazh_2014_13_20

  49. Ahuja, M.R., Polyploidy in gymnosperms: revisited, Silvae Genet., 2005, vol. 54, no. 2, pp. 59–69. https://doi.org/10.1515/sg-2005-0010

    Article  Google Scholar 

  50. Hizume, M., Tominaga, K., Kondo, K., Gu, Z., and Yue, Z., Fluorescent chromosome banding in six taxa of Eurasian Larix, Pinaceae, Kromosomo II, 1993, vol. 69, pp. 2342–2354.

    Google Scholar 

  51. Hizume, M., Kuzukawa, Y., Kondo, K., Yang, Q., Hong, D., and Tanaka, R., Localization of rDNAs and fluorescent bandings in chromosomes of Larix potaninii var. macrocarpa collected in Sichuan, China, Kromosomo II, 1995, vol. 78, pp. 2689–2694.

    Google Scholar 

  52. Liu, B., Zhang, S.-G., Zhang, Y., Lan, T.-Y., Qi, L.-W., and Song, W.-Q., Molecular cytogenetic analysis of four Larix species by bicolor fluorescence in situ hybridization and DAPI banding, Int. J. Plant Sci., 2006, vol. 67, no. 2, pp. 367–372. https://doi.org/10.1007/s11295-009-0258-1

    Article  Google Scholar 

  53. Ahuja, M.R. and Neale, D., Evolution of genome size in conifers, Silvae Genet., 2005, vol. 54, no. 3, pp. 126–137. https://doi.org/10.1515/sg-2005-0020

    Article  Google Scholar 

  54. Nkongolo, K.K. and Mehes-Smith, M., Karyotype evolution in the Pinaceae: implication with molecular phylogeny, Genome, 2012, vol. 55, no. 10, pp. 735–753. https://doi.org/10.1139/g2012-061

    Article  CAS  PubMed  Google Scholar 

  55. Peruzzi, L., Leitch, I.J., and Caparelli, K.F., Chromosome diversity and evolution in Liliaceae, Ann. Bot. (Lond.), 2009, vol. 103, no. 3, pp. 459–475. https://doi.org/10.1093/aob/mcn230

    Article  CAS  Google Scholar 

  56. Mathew, P.M., Mathew, P.J., Christopher, C., and Haridas, P., Karyomorphological studies in conifers, J. Cytol. Genet., 2014, vol. 15 (NS), pp. 107–121.

  57. Murray, B.G., Karyotype variation and evolution in Gymnosperms, Plant Genome Diversity, 2013, vol. 2, pp. 231–243. https://doi.org/10.1007/978-3-7091-1160-4_14

    Article  Google Scholar 

  58. Eckenwalder, J.E., Conifers of the World, OR, Portland: Timber Press, 2009.

    Google Scholar 

  59. Rodionov, A.V., Polyploidy and interspecific hybridization in the evolution of flowering plants, Vavilov. Zh. Genet. Selekts., 2013, vol. 17, nos. 4/2, pp. 916–929.

  60. Kalashnik, N.A., Chromosome aberrations as indicator of estimation of degree of technogenic effect on conifer plantations, Russ. J. Ecol., 2008, no. 4, pp. 276–286.

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Sedel’nikova, T.S., Pimenov, A.V. Karyological Study of Siberian Larch Species Larix sibirica and Larix gmelinii in Taimyr. Cytol. Genet. 53, 202–211 (2019). https://doi.org/10.3103/S0095452719030046

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